Enzymes and Inhibitors
New England Biolabs, Inc. HindIII-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BamHI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Accelagen 1000U HRV3C PROTEASE GST/HIS
Turbo3C Protease is the most user-friendly HRV3C protease and offers the advantage of a dual His/GST tag. Turbo3C is a high specific restriction grade protease with robust activity and excellent stability. It recognizes the PreScission site of LEVLFQGP and cleaves between Q and G.
AVIDITY LLC BIRA500-RT Biotin Protein Ligase, 2 vials of 20 μL each, 40 μg total, Lyophilized reaction kit, Covalent addition of d-biotin via ATP, >99% purity
The BIRA500-RT Biotin Protein Ligase is a lyophilized reaction kit designed for efficient and targeted biotinylation. This process is crucial in various applications such as affinity chromatography, protein immobilization, and detection with anti-biotin antibodies or avidin conjugates. Optimized for use with AviTag™ peptides, the kit enhances reaction rates, reduces enzyme use, and shortens incubation times, minimizing issues related to proteases and protein instability.
- Enzyme name: BirA biotin-protein ligase
- EC number: 6.3.4.15
- Form: Lyophilized reaction kit
- Source: E. coli wild-type
- Purity: >99%
- Molecular weight: 33,500 Daltons
- Activity: >8000 Units/μg of BirA
- Recommended substrate: AviTag™ peptide sequences
- Stored lyophilized: 1 year at 4°C
- Reconstituted storage: 2 weeks at 4°C, long term at -80°C
- Price: $250.00
Sigma Aldrich Fine Chemicals Biosciences Catalase from bovine liver | 9001-05-2 | MFCD00081483 | 100 mg
Catalase from bovine liver | Mol Wt: tetramer ~250 kDa | 9001-05-2 | MFCD00081483 | 100 mg
Surmodics Inc TMB Super Sensitive One Component HRP Microwell Substrate, 100mL
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
Iwai North America IMATRIX-511 1050UG 175UG X6
iMatrix-511 is a recombinant Laminin- 511 E8 fragment expressed by CHO-cell. Laminin-511 E8 fragments allow ES/iPS cells and MSCs to be maintained in xeno-free culture conditions. It also enables the passaging of single cells and provides greater adhesion than full-length Laminin, Vitronectin, or Matrigel.
Worthington Biochemical Corporation DNASE 11MG FILTER
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES BENZONASE SAFETY PLUS 100KU
NC3315747 BENZONASE SAFETY PLUS 100KU
Worthington Biochemical Corporation DNA'ASE 5MG
Deoxyribonuclease I, Chromatographically Purified, Equivalent to 1X Crystallized Dnase, A Lyophilized Powder with Glycine as Stabilizer, Store at 2 deg. to 8 deg.C, Protect from Moisture, 5mg
Sigma Aldrich Fine Chemicals Biosciences Alpha-Amylase from porcine pancreas | MFCD00081319 | 1MU
Alpha-Amylase from porcine pancreas | MFCD00081319 | 1MU
Enzyme Research Laboratories Inc HUMAN FACTOR ALPHA XIIA 0.5MG
Human Factor alpha -XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3915858 A-AMYLASE FROM HUMAN SALIVA
Seracare Life Sciences Inc TMB SUBSTRATE SOL B 1X100ML
Most sensitive chromogenic substrate for peroxidase detection, more sensitive than ABTS and OPD. It develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm. Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA and blotting applications. 2-component format of 3, 3', 5, 5' - Tetramethylbenzidine, simply mix and use. Has longer shelf life than 1-component TMB substrates.